Funding for Access
France-BioImaging is the French Node of the European research infrastructure Euro-BioImaging.
As such, France-BioImaging invites all its external users* to register through Euro-BioImaging web portal to access any equipment available on France-BioImaging facilities and labs.
By registering through the EuBI web portal, France-BioImaging external users will:
- have access to 23 biological imaging facilities and laboratories specializing in R&D for imaging in 8 regional nodes
- receive a waiver for the costs of instrument access (1) (up to 750€ per week) on FBI facilities (2).
- have access to a wide range of technical and methodological expertise: Single Particle Tracking & Super Resolution / Multimodal & Quantitative fluorescence Microscopies / Multiscale & Correlative Microscopy / New Contrast & In-Depth Imaging / High Throughput & High Content Screening / Probe development, Optomanipulation & Optogenetics / BioImage Informatics, Image Processing & Data Management
- receive a strong support in computational analysis, provide quantitative measures and integrative understanding of a wide range of cell and tissue activities in biological models, from the simplest, to small animals in normal and pathological situations
Submit a proposal
To Request Access to France-BioImaging services, external users have to fill out a simple online form on the Euro-BioImaging Web portal to create a research proposal.
Timeframe: The application and review process is efficient, transparent and quick. The whole process is estimated to take 2-3 weeks.
When to apply: Applications for access can be submitted at any time. Periodically, special calls for access will be published with a defined deadline. These special calls carry specific criteria, so please read the calls carefully.
Proposal application steps:
- Go to the Euro BioImaging website: https://www.eurobioimaging.eu/service, click on “biological Imaging”, select the technology that will be used and the host node (French BioImaging Node). Then you will have to click on “select” (available service section).
- “Name_of_the_Technology @ France – French BioImaging Node” will appear and you will have to click on “make a proposal”. See the list of technologies available below.
- You will then be redirected to the Life Science Login page to create an account and register your project. You should find your home institution in the institution list, otherwise there are alternatives (Google, ORCID, etc.)
- Submit your proposal: Once you are logged in through Life Science Login, fill in and submit the simple proposal form (see https://www.eurobioimaging.eu/about-us/how-to-access for an overview of the form). NB: It is important to highlight the imaging aspects of the project and their importance, as well as to mention that contact has already been established with the France BioImaging facility/lab and the name of your contact on site. Also, if the project has already undergone an external scientific evaluation (i.e. for a grant) it is important to mention it, since it will greatly speed up the acceptance by Euro BioImaging.
*This registration process is open to:
- any users from outside the institutional perimeter of France-BioImaging nodes (i.e. from outside the following institutions: Aix-Marseille Université, Université de Montpellier, Université de Bordeaux, Université de Nantes, Université de Rennes 1, Université Paris-Saclay, Université de Paris, Université PSL -Paris Sciences & Lettres-, Généthon, Ecole Polytechnique, Institut Pasteur) who would like to use imaging technologies in one of FBI nodes: Paris Centre, Paris Ile-de-France-Sud, Marseille, Montpellier, Bordeaux, Bretagne-Loire. They can be French or international users – EU and non-EU
- or users from one France-BioImaging regional Node who want to access an equipment available in another FBI regional node.
Questions? Contact us: France BioImaging coordination team
Offered technologies @France – French BioImaging Node
| Deconvolution widefield microscopy (DWM) | ✓ |
| Laser scanning confocal microscopy (LSCM/CLSM) | ✓ |
| Spinning disk confocal microscopy (SDCM) | ✓ |
| Structured illumination microscopy (SIM)* | ✓ |
| Total internal reflection fluorescence microscopy (TIRF) | ✓ |
| Two-photon microscopy (2P) | ✓ |
| Objective-couple planar illumination (OCPI) | ✓ |
| Image Scanning microscopy (ISM) | ✓ |
| Random Illumination Microscopy (RIM)* | ✓ |
| Lattice Lightsheet (LL) * | ✓ |
| Single Molecula localisation microscopy (SMLM) | ✓ |
| Stimulated emission depletion microscopy (STED) | ✓ |
| Reversible optical fluorescence transitions (RESOLFT) | ✓ |
| Light-sheet mesoscopic imaging (SPIM or dSLSM) | ✓ |
| Optical projection tomography (OPT) | ✓ |
| Macro Serial Block face Fluorecence imaging* | ✓ |
| Raman Spectroscopy (RS) | ✓ |
| Quantitative Phase Imaging* (QPI) | ✓ |
| Polarization microscopy (PM) | ✓ |
| Second/Third Harmonics Generation (SHG/THG) | ✓ |
| High throughput microscopy/high content screening (HTM/HCS) | ✓ |
| Fluorescence (cross)-correlation spectroscopy (FCS/FCCS) | ✓ |
| Fluorescence Resonance Energy Transfer (FRET) | ✓ |
| Fluorescence Recovery After Photobleaching (FRAP) | ✓ |
| Fluorescence Lifetime Imaging Microscopy (FLIM) | ✓ |
| Intravital Microscopy (IVM) | ✓ |
| Voltage/pH/Ion Imaging * | ✓ |
| Microdissection * | ✓ |
| High-speed Imaging * | ✓ |
| Imaging at Biosafety Level >1 | ✓ |
| Photomanipulation | ✓ |
| Anisotropy/Polarization Microscopy | ✓ |
| Expansion Microscopy * | ✓ |
| Feedback microscopy * | ✓ |
| Multiplexing imaging *(Codex, Opal, Celldive) | ✓ |
| Tissue Clearing (TC)* | ✓ |
| Single molecule FRET * | ✓ |
| TEM of chemical fixed samples (TEM) | ✓ |
| TEM of cryo-immobilized samples (TEM cryo samples)* | ✓ |
| Large scale EM | ✓ |
| EM tomography (ET) | ✓ |
| serial section TEM | ✓ |
| Serial Blockface SEM | ✓ |
| STEM tomography | ✓ |
| Array tomography | ✓ |
| Immuno-gold EM on thawed cryo-sections (Tokuyasu-EM) | ✓ |
| Immuno-gold EM on resin sections (resin-EM) | ✓ |
| Pre-embedding immunolabelling (pre-embed IL) | ✓ |
| Genetic encoded EM probes | ✓ |
| Pre-embed CLEM | ✓ |
| on-section CLEM | ✓ |
| Cryo Electron Tomography (Cryo-ET)* | ✓ |
| Cryo Transmission Electron Microscopy (Cryo-TEM)* | ✓ |
| Scanning Electron Microscopy (SEM) | ✓ |
| pre-embeded CLEM | ✓ |
| in-section CLEM | ✓ |
| live-cell CLEM | ✓ |
| CXEM (Correlative X-ray and EM) * | ✓ |
| in vivo optical imaging | ✓ |
| Traction Force Microscopy (TFM) * | ✓ |
| Atomic Force Microscopy* (AFM) | ✓ |
| Image Analysis-bio * | ✓ |
Questions about the available technologies and technical feasibility should be addressed directly to the heads of the France BioImaging facilities.
General conditions
Waiver of costs
(1)The following costs are eligible for financial support:
- Contribution to the verifiable incurred costs for measurement – data acquisition
- Imaging related sample preparation (special chemicals, labels, etc.)
- Data processing and analysis
France BioImaging financial support will be transferred directly to the France BioImaging core facility/lab in which the project will be performed. The support does not include travel costs, accommodation and daily allowances. This has to be covered by the user.
(2)Restrictions: This waiver does not apply for users from Industry.
Acknowledgements
Publications resulting from work undertaken at France Bioimaging facilities/labs have to contain the following acknowledgement:
“The National Infrastructure France-BioImaging supported by the French National Research Agency (ANR-10-INBS-04)”
Depending on the context, we suggest three model sentences:
- For the publications of scientists using one of the France-BioImaging Imaging Facilities: “We acknowledge the X (name of imaging facility), member of the national infrastructure France-BioImaging supported by the French National Research Agency (ANR-10-INBS-04)”
- For the publications by FBI members (technological development or scientific projects and collaborations) the sentence must appear in tributes as follows: “We acknowledge France-BioImaging infrastructure supported by the French National Research Agency (ANR-10-INBS-04)
- For the FBI external users (EuBI users) whose access to FBI facilities/lab was subsidized by France-BioImaging: “The study was supported by the France-BioImaging User Access Fund”